Under the influence of sub-bacteriostatic doses of novoimanin on agar two types of colonies of staphylococcus arose – normal size and forms, consisting of the usual cell size, and in a larger number – dwarf colonies consisting of larger cells – round, oval, rod-shaped, solitary, pairwise or small groups unevenly colored; Streptococcus also lost their inherent location, they are larger, their tinctorial properties are changed.
In Bacillus subtilis, changes in the shape of colonies are more noticeable, and in Lactobacterium Plantarum (not in all strains) – cell forms. All these phenomena are less pronounced than, for example, in the same bacteria under the influence of penicillin, but still very vivid. Pseudomonas aeruginosa in the presence of novoimanin in the medium grew in the form of small bespigamentnyh colonies. It is interesting to note the coincidence of these data with the observations of PS Volosovets (1964), who noted the loss of pigment by the Pseudomonas aeruginosa, isolated from patients treated with a new Lyman.
Morphological changes in bacteria under the influence of the new immanin were also found in other species of bacteria, especially in the presence of serum in the medium. The change in the morphology of cells under the influence of the new immanin depends on the nature of the medium and the amount of the preparation in it, the time and conditions of contact. But even when the influence of these factors is taken into account, a certain regularity is revealed: the newiman causes an increase or decrease in cell sizes, a change in their tinctorial properties; in the study of the majority (only Gram-positive species), a decrease in the chromatin substance.
Novoimanin, according to GI Poroshina (1964), causes inhibition of acid formation in lactic acid bacteria. The active acidity of the medium with their growth in the presence of subbacteriostatic doses of the antibiotic in the medium increases more slowly than without it. The fermenting properties of Leuconostoc mesenterioides, Streptococcus lactis, Lactobact also change . plantarum; on a medium with an antibiotic, more acetic acid is observed than in a medium without a preparation, i.e., a new immanin causes a shift in the fermentation process of lactic acid bacteria towards heterofermentation. Novoimanin inhibits the rate of dehydrogenation of glucose, galactose, fructose, sucrose, arabinose, xylose, mannitol, sorbitol, alcohol, α- and β-alanine, leucine in L eucopo sto with те s е nter ioides, and inStreptococcus lactis only glucose, lactose, sucrose, maltose, sorbitol, in Lactobact. r1ap1a rum α-alanine. Differences in the character and degree of influence of the new Lyman on the dehydrogenation of substrates of not only different species but also strains of lactic acid bacteria are noted.